Contents
Protocol for in vitro embryo generation in goat (1/2)-Practical
Dr. BP Mishra, HD, Biotech
Amplification of a transgene by PCR (Practcal)
Dr Deepak Sharma, HD, AG
Detection of transgene expression by SDS PAGE and Western blotting-(Practical)
Dr. S Patra, HD
In Vitro Generation of Buffalo Pluripotent Embryonic Stem Cells for the Production of Transgenic Embryos - (Practical)
Dr. Ran Vir Singh, Sr Sci, AG
Insilico Analysis of a recombinant Gene Expression
Dr Praveen Gupta
Fertility Status of Transgenic Animal - Theory
Dr Tarun Bhattacharya
Transgenic RNAi in Livestock and Poultry
Dr. Mnishi Mukesh, NF, NBAGR
Promoters: Driver for Tissue Specific Expression (Theory)
Dr. Anuj Chauhan, Sci, AG
Isolation of genomic DNA and RNA from bovine sperm(Practical)
Dr Taru Sharma, HD, P&C
Total RNA isolation from tissue and cDNA synthesis (Practical)
Dr Yash Pal
Reversal of cell fate decision for generation of induced pluripotent stem cells-(Theory)
Dr. Bhaskar Sharma, Natl Prof
Somatic cell transgenesis (gene therapy) for cancer remedial - (Lecture)
Dr. Sadhan Bag, PS, P&C
Flow Cytometry and its application in biological science-(Practical)
Dr. A. K. Tiwari, HD, Stanadarization
Characterization of cells by immunocytochemistry - (Practical)
Dr. Sanjeev Bhure, Sr Sci, Biochem
Sperm Mediated Gene Transfer (SMGT): A potential way to produce transgenic chicken-(Lecture)
Dr. Bharat Bhushan, PS, AG
Integration of exogenous DNA into chicken spermatozoa and its verification - (Practical)
Dr. Pushpendra Kumar, PS AG
Protocol for in vitro embryo generation in goat (2/2)-Practical
Dr. Sujoy Dhara, Sr. Sci, Biotech
Testes mediated gene transfer- A tool for producing transgenic animals - (Lecture)
Dr. Amit Kumar, Sci, AG
Stem cell technology and its use in the production of transgenic animals - (Lecture)
Dr. Praveen Singh, PS, Biophysics
Trasgenic research in poutry - (Lecture)
Dr. Sameer Srivastava, Sr Sci, Biotech
Gene cloning in TA cloning vector (Practical)
Dr Satish Kumar, Dean, NIAB
RealTime PCR: Priniciple and chemistry - (Lecture)
Dr. Arvind Sonwane, Sci, AG
Lentiviral mediated gene delivery-Theory
Dr. S Mishra, HD, P&T
Lentiviral mediated gene transfer in mammalian cells-Practical
Prof Gaya Prasad, Director
Breeding and maintenance of transgenic animals-Theory
Dr Bikas Mondal, PS
Fluorescent in situ hybridization (FISH): A technique for zygocity evaluation in transgenic animals - Theory
Dr. RP Singh, I/C, ITMU
Global gene expression analysis using microarray techniques - (Practical)
Dr. Sanjay Kumar, I/C ARIS
Protocol for Real time PCR using SYBR green-(Practical)
Dr. Bhanja. CARI
Sperm mediated gene transfer in goat and assessment of semen parameters after electroporation - Practical
Dr. Khitish Mazumdar, Former Sci
Isolation and culture of caprine seminiferous tubules - Practical
Dr. N Sahoo, Sci, AG
Database management system - Theory
Dr. Amit Kumar
Genetic Manipulation of Mesenchymal Stem Cells - Practical
Dr. Subodh Kumar
Demonstration of cell penetrating property of delivery peptides: One of the pre-requisite for developing any synthetic peptide based gene delivery system - Practical
Dr. Abhijit Mitra
Marker Assisted Introgression of Fecundity (FecB) gene in sheep - Theory
Dr. Abhijit Mitra
Isolation and culture of Spermatogonial stem cells (SSCs) in goat - Practical
Dr. Aditya Sahoo.
RNAi mediated transgenesis for improving animal produce - Theory
Dr. N. R. Sahoo
Urothelial cell culture - Theory
Dr. Anuj Chauhan
Assessment of promoter strength through Dual-glow luciferase assay - Theory
Dr. Anuj Chauhan
Statistical Analysis Using SAS - Practical
Dr. Sujoy Dhara
Immunocytochemistry: Principles and Methods -Practical
Dr. GVPPS Ravi Kumar
Application of cytogenetic tools in transgenic technology - Theory
Dr. Arvind Sonwane
Development and evaluation of synthetic cell penetrating peptides for targeted gene delivery-Theory
Dr. Arvind Sonwane
Cationic lipid-mediated transfection of the cells - Practical
Dr. Abhijit Mitra
Demonstration of Assessment of Apoptosis-Practical
Dr Abhijit Mitra
Intellectual Property Rights: Issues in Transgenic Research-
Dr. Abhijit Mitra
Targeted transgenesis: integrating transgene at a predetermined genomic locus -Theory
Dr. Sameer Srivastava
Confocal microscopy: Principals and applications in transgenesis research - Practical
Dr. Arvind Sonwane
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Summer School

Recent advances in Transgenesis in livestocks
22 May 2014 - 11 Jun 2014

E-BOOK


Edited By
Dr. Abhijit Mitra
Dr Arvind Sonwane
Dr Yashpal Singh

E-Manual Designed and Developed By
Division of Computer Applications
Indian Agricultural Statistics Research Institute,
Library Avenue, Pusa,
New Delhi - 110 012 (INDIA)